ࡱ> 352  bjbjҬҬ 4"Ɯ_Ɯ_` Yxxxxx8$F$|xxx   Rxx    Gb~) 0F "" "x  cF"X >: Labware Cleaning Protocol Accuracy and repeatability in our analytical measurements depends on the availability of properly cleaned labware. It is very important to us all that EVERY lab participant washes the equipment that they have used to the same stringent standards. It is also important that each participant takes responsibility for cleaning up their glassware, and any dirty bench space that they have used, AS SOON AS POSSIBLE after the analyses are complete. This responsibility includes storing away the glassware after washing so that it is available to the next user. Tips/Requests: a) Prior to washing, keep dirty glassware and sample cups wet by filling with water and storing in a water-filled basin so that dirt is not engrained onto surfaces prior to washing. b) Never leave dirty labware in the sinks. If necessary, they should be left to soak in a separate water-filled basin identified with your name. c) Rubber and steel should NOT be put into the acid bath, they will deteriorate and/or rust. d) Pipette tips that have been used to measure out wetting agents (BRIJ 35, AEROSOL 22) should not be put into the acid bath. Cleaning these residues off is difficult. Instead, place such tips in the garbage. Washing procedure: 1. Rinse once thoroughly with hot tap water. 2. Use soap (Fisherbrand Versa Clean) to clean pieces if have been working with carbon-based chemicals such as solvents (chloroform, phenols, formaldehyde) etc., plants residues or soils (i.e. K2SO4 soil extracts). If soap is NOT required proceed to step 5. 3. Use brush to scrub out equipment. The main goal is to remove all visible residue. 4. Rinse 3 times with hot tap water. 5. Put into acid bath (1% HCl) and leave overnight. One night is enough. To avoid holding up other users, please remove labware from the acid bath as soon as possible thereafter. ***NOTE*** Even though 1% is a weak acid it will still bleach clothing and irritate skin and eyes. You should wear heavy gloves (i.e. rubber washing up gloves, not just Latex ones), lab coat, and goggles when working with the acid bath to reduce chance of accidents. The acid solution will corrode steel and so should not be allowed to come in contact with the steel sinks. 6. Remove each piece from the acid bath and rinse it under tap water in the stone sink. 7. Move pieces from the stone sink to the metal sink using a plastic basin and thoroughly rinse them with reverse osmosis water (from the lab DIW tap), and then finally briefly immerse each piece in a clean basin of DISTILLED water. 8. Set out to dry 9. Put away when dry Acid Bath Recipe: The acid bath is composed of a 1% HCL solution. It should be changed every 6 months. To make a new solution 10mL of HCl is added to 1L of solution. For example, to make a 20L acid bath, add 200mL of concentrated (12 M) HCl to 800ml of reverse osmosis water (from the lab DIW tap) in the fumehood (wearing coat, face mask, appropriate elbow-length gloves). Note: to avoid dangerous boiling/bubbling never add water to concentrated acid. Always measure out the water first into a large enough container, and then add the acid. Then, add this solution to 19L of reverse osmosis water (from the lab DIW tap) in the acid bath container. Stir mixture with glass pipette and then leave area until fumes settle. Mark date acid was changed on some duct tape and stick to the bath lid.     Appendix 1. Protocol 1. 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J ` ` b c c e f h i k l    . J ` ` b c c e f h i k l $#W;-(4 DeDwK\QNT`Mj]ngAO3}/* 1byLM(T2K6C '<h ` b @    @UnknownG.[x Times New Roman5^Symbol3. *Cx Arial5. .[`)TahomaC.,.{$ Calibri Light7..{$ CalibriA$BCambria Math"qhgKFa a x24Z Z 3Q HP ?(42!xxk` Cleaning Protocol Alison Fidler Paul Grogan Oh+'0  , L X d p|'Cleaning ProtocolAlison Fidler Normal.dotm Paul Grogan8Microsoft Office Word@~@@ۺ@&/b~a  ՜.+,D՜.+,L hp  ' Queens UniversityZ  Cleaning Protocol Title@@ 4_AdHocReviewCycleID_EmailSubject _AuthorEmail_AuthorEmailDisplayName_ReviewingToolsShownOnce'+Safety and Protocols.groganp@biology.queensu.ca Paul Grogan  !#$%&'()+,-./014Root Entry F]Gb~61Table"WordDocument4"SummaryInformation("DocumentSummaryInformation8*CompObjr  F Microsoft Word 97-2003 Document MSWordDocWord.Document.89q